Thus, using ehrlichs reagent containing hcl in the hydroxyproline assay represents an advance in both mitigating laboratory safety hazards and improving biochemical collagen quanti. All samples and standards should be run in duplicate. A modified hydroxyproline assay based on hydrochloric acid. Assay protocol for the colorimetric detection of hydroxyproline in biological samples using the hydroxypoline assay kit. Hydroxyproline assay kit mak008 technical bulletin. Featuring a simple and quick protocol, this hydroxyproline assay yields a. Hydroxyproline assay kit perchloratefree clinisciences. Enzymatic method for determining hydroxyproline sigmaaldrich. Hydroxyproline in tissue hydrolysates can be used as a direct measure of the amount of collagen present. Hydroxyproline is formed posttranslationally from specific proline residues by action of the enzyme prolylhydroxylase. Fast and sensitive collagen quantification by alkaline hydrolysis hydroxyproline assay. For uncooked samples, place 6 g of sample in weigh pan, record weights and heat in 110 c oven for 1. A modified hydroxyproline assay based on hydrochloric acid in. Overview hydroxyproline assay kit colorimetric ab222941 provides a quick and convenient method to detect hydroxyproline in tissue lysates as well as biological fluids such as urine and serum.
K226100 hydroxyproline assay kit perchloratefree v3 biovision. The quickzyme hydroxyproline assay is a modification of the method described by prockop and udenfriend anal. It is advised to only make as much reagent as is necessary for the number of samples and standards to be assayed. It is found only in collagen and elastin in mammals but exists in a number of other proteins in plants. Rearrange individual pages or entire files in the desired order. The linear range of detection for this assay is between. Biovisions hydroxyproline assay kit ii perchloratefree uses a proprietary acidic. Buy hydroxyproline assay kit mbs169258 product datasheet at mybiosource, assay kits. Hydroxyproline 4hydroxyproline, hyp is a common nonproteinogenic amino acid. Enzymatic method for determining hydroxyproline sigma. In addition, hydroxyproline measurement has been used to identify certain diseases that involve breakdown of collagen. Hydroxyproline is formed only as a posttranslational modification in the peptide chain and proline hydroxylase does not hydroxylate free proline.
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